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WhoGA チュートリアル
[はじめに]
  1. ここに記載した Tutorial は WhoGA 依存の部分もありますが、GBrowe上では、他のサイトでも同様の操作が出来ます。GBrowse の説明は、[annotation 画面の説明] にあります。

[main 画面の説明]
当サイトでは、どの画面でも以下の様な操作画面が存在します。

  1. 図の下の部分にあるタブはクリックするとそれぞれ Top => main 画面に戻る。
    What's New => 更新情報。
    GBrowse => annotation 結果を表示させる。
    Download => 各種公開データのダウンロード。
    Link => 関連情報。
    へアクセス出来ます。

  2. 右上方の染色体図は、それぞれの染色体のアノテーション画面へのショートカットです。クリックされた凡その位置を GBrowse で表示します。

  3. 左上の検索窓は rgp.dna.affrc.go.jp サイト内検索となります。

  4. アノテーション手法について知りたければ、main 画面左の About RGP annotation にアクセスしてください。

  5. 数値的な情報に関しては Data Collection にアクセスしてください。

[annotation 画面の説明] - GBrowse の説明
では、GBrowse をクリックしてみてください。これがアノテーション結果閲覧画面となります。一回もアクセスしてなかった場合は、絵が出てこないと思います。説明セクションの「例」をクリックしてください。

上から順に説明します。

  1. 説明文です。必要無ければ左上の + ボタンをクリックして非表示としてください。以下、どのセクションでも同じです。


  2. GBrowse 上での検索、出力を担うセクションです。

    2.1

    という検索窓にて、染色体上の任意の場所にアクセス出来ます。
    • chrXX:start..end という書式にて位置情報からアクセス出来ます。 例) chr12:100..4000
    • RGP の EST maker name, RFLP maker name にて位置情報とは無関係にアクセス出来ます。例) C0562A
      ※マーカー名は正しく入力してください。
      -- 複数箇所に有意な結果を示す場合は、選択画面が表われますのでお好みの結果をクリックしてください。
    2.2

    以下のファイルを出力出来ます。
    • GFF file
    • fasta sequence
    2.3

    アノテーション画面のスクロール/ズーム、位置移動が出来ます。
    - 一画面左方に移動
    - 半画面左方に移動
    - ズームダウン
    - 詳細ビューの表示単位を変更します。
    - ズームアップ
    - 半画面右方に移動
    - 一画面右方に移動
    以上は、操作が直ぐに反映されます。

    - 詳細ビューの表示の反転です。
    直ぐには反映されませんのでチェックボックスクリック後、上記何れかの操作をするか、 後述の詳細ビューにて「画像を更新」をクリックしてください。


  3. 染色体全体から見た、閲覧している位置を赤枠で示します。


  4. よりズームアップした領域での位置を赤枠で示します。


  5. ここにアノテーション結果等が表示されます。各項目をトラックと呼びます。
    5.1位置情報

    位置情報を示します。
    5.2BAC

    アノテーションに使用した BAC / PAC の地図上の位置を青いボックスで示します。ボックスをクリックすると、位置情報、長さ、NCBI の当該クローンへのリンクが表われます。
    5.3Predicted Genes.

    我々のアノテーションの結果予測された遺伝子等が表示されます。拡張子 の有無によってタイプが分れますので、以下の説明を参考にしてくださ い。詳しくは[About RGP Annotationの3. 遺伝子機能予測]以降を参考に してください。
    • 拡張子なし => 予測遺伝子。IRGSP基準に基き、予測プログラム2つ以上で支持されているか、それ以上に有意であると判断されているもの。
    • .spp => 一つの予測プログラムからの予測。予測遺伝子としては採用してい ない。
    • .nc => 完全長 cDNA にて遺伝子の存在が示唆されたものの、遺伝子を組む事が出来なかった領域。
    • .pseudo => 終止コドンやフレームシフトが原因で、遺伝子が機能していないと思われる領域。
    ボックスをクリックすると、位置情報、領域、NCBI への hit した完全長 cDNA のリンク等が表われます。
    5.4NR hits

    既知遺伝子が相同性を示した領域を表示。ラインをクリックすると当該領域の上位数個の情報が表われます。NCBI へのリンクもありますので行ってみてください。なお、既知遺伝子は、「アノテーション時点」の物を表示しております。現行のデータベースを直接反映したものではありません。
    5.5full length cDNA

    「アノテーションに使用した」完全長 cDNA を表示します。
    5.6ESTs

    「アノテーションに使用した」EST marker を表示します。
    5.7Rice RFLP marker

    「アノテーションと無関係に」当該領域に位置付けられた RFLP marker を表示します。位置情報認識の指標としてご利用ください。
    右下の「画面を更新」クリックボックスにて画面の更新が出来ます。画面が更新されない場合にご利用下さい。


  6. このセクションにて各トラックの表示設定の有無が出来ます。
    6.1. Category 1 RGP Annotation アノテーションに関連したトラックの表示の有無が選択出来ます。

    6.2. Category 2 Marker その他の公開されている marker の表示選択が出来ます。
    右下の「画像を更新」クリックボックスにて反映させてください。
以降の 2 セクションに関しては、GBrowse の[ヘルプ]を参考にしてください。

以上、ざっとですが基本的な部分を列挙しました。


[topへ戻る]


 

rgpwebmaster @ml.affrc.go.jp
Copyright (C) Rice Genome Research Program (RGP). 2007 All rights reserved.
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chr6:1500001..1520000 go gbrowse chr6:2000001..2020000 go gbrowse chr6:2500001..2520000 go gbrowse chr6:3000001..3020000 go gbrowse chr6:3500001..3520000 go gbrowse chr6:4000001..4020000 go gbrowse chr6:4500001..4520000 go gbrowse chr6:5000001..5020000 go gbrowse chr6:5500001..5520000 go gbrowse chr6:6000001..6020000 go gbrowse chr6:6500001..6520000 go gbrowse chr6:7000001..7020000 go gbrowse chr6:7500001..7520000 go gbrowse chr6:8000001..8020000 go gbrowse chr6:8500001..8520000 go gbrowse chr6:9000001..9020000 go gbrowse chr6:9500001..9520000 go gbrowse chr6:10000001..10020000 go gbrowse chr6:10500001..10520000 go gbrowse chr6:11000001..11020000 go gbrowse chr6:11500001..11520000 go gbrowse chr6:12000001..12020000 go gbrowse chr6:12500001..12520000 go gbrowse chr6:13000001..13020000 go gbrowse chr6:13500001..13520000 go gbrowse chr6:14000001..14020000 go gbrowse chr6:14500001..14520000 go gbrowse chr6:15000001..15020000 go gbrowse chr6:15500001..15520000 go gbrowse chr6:16000001..16020000 go gbrowse chr6:16500001..16520000 go gbrowse chr6:17000001..17020000 go gbrowse chr6:17500001..17520000 go gbrowse chr6:18000001..18020000 go gbrowse chr6:18500001..18520000 go gbrowse chr6:19000001..19020000 go gbrowse chr6:19500001..19520000 go gbrowse chr6:20000001..20020000 go gbrowse chr6:20500001..20520000 go gbrowse chr6:21000001..21020000 go gbrowse chr6:21500001..21520000 go gbrowse chr6:22000001..22020000 go gbrowse chr6:22500001..22520000 go gbrowse chr6:23000001..23020000 go gbrowse chr6:23500001..23520000 go gbrowse chr6:24000001..24020000 go gbrowse chr6:24500001..24520000 go gbrowse chr6:25000001..25020000 go gbrowse chr6:25500001..25520000 go gbrowse chr6:26000001..26020000 go gbrowse chr6:26500001..26520000 go gbrowse chr6:27000001..27020000 go gbrowse chr6:27500001..27520000 go gbrowse chr6:28000001..28020000 go gbrowse chr6:28500001..28520000 go gbrowse chr6:29000001..29020000 go gbrowse chr6:29500001..29520000 go gbrowse 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